Contact Us Add to Favorite
 
NianQi Search Adv Search
33
研究论文 Current Issue | Archive | Adv Search |
An Improved Method for The Culture of Mouse Zona-Free Embryos
Download: PDF (0 KB)  HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  Abstract: To search for an effective culture method for zona-free embryos and provide technical support for handmade cloning, this study displayed three methods for the culture of zona-free embryos and improved the more effective well of the well (WOW) culture method that embedded the well with calcium alginate gel and tried to combine with the co-culture technology. The results indicated that the blastocyst development rate(54.7 %) and the average cell numbers of blastocysts (54.3) culturing with calcium alginate gel embedding well(AEW) culture method was significantly higher than culturing with the micro-drops single culture method and agarose-pillar embedding culture method ( P<0.05) and there was no significant differences (P>0.05) with the WOW culture method(P>0.05); This study also found the cleavage rate and the blastocyst development rate culturing with this new culture method were no significant differences(P>0.05) when the concentration of the sodium alginate was used between 0.5 %~1.0 % and the concentration of the calcium ion was used between 1.0 %~2.0 %, and the optimal concentration of the sodium alginate and calcium ion were 0.7 % and 1.5 %; The study also found the cleavage rate(89.7 %) and blastocyst development rate (67.9 %) culturing with the AEW culture method combining with co-culture technology was significantly higher than culturing with the AEW culture method alone (P<0.05). The whole results indicated that the effect of AEW culture method on culturing zona-free mouse PN-stage embryos was better than the other culture methods; this method combining with granule cells co-culture technology could greatly raised the cleavage rate and blastocyst development rate for the culturing of zona-free embryos..In conclusion,this method is suitable for the batch zona-free cloned embryo production.
Key wordsmouse      the culture of zona-free embryos      calcium alginate gel embedding well culture method     
Received: 08 August 2007     
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Cite this article:   
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2008/V16/I2/0
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech