|
|
|
| Experimental research on cryopreservation of boar semen using 0.25 mL straws |
| Rong RUI |
| |
|
|
|
|
Abstract The purpose of this study is to establish an optimized protocol to cryopreserve boar semen. The results showed that the optimal procedure should be conducted as follows. Firstly, boar semen were pre-diluted with ZO solution and pre-equilibrated at room temperature for 1 h. After adding extender I, spermatozoa were equilibrated at 5℃ for 1.5 h and then the equal volume of extenderⅡ was added, holding 2 h for equilibration. The resulting spermatozoa were loaded into 0.25 mL straws and equilibrated for 10 min at 3 cm above the surface of liquid nitrogen (LN) and then put into LN promptly. When thawing, straws were put into water bath at 37℃ for 30 s, resulting in optimal post-thaw motility of 0.58.
|
|
Received: 02 September 2007
|
|
Corresponding Authors:
Rong RUI
|
|
|
|
|
|
|
