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| Mutation Breeding of Aspergillus niger LW-1 for Producing High-yield Acidic β-Mannanase |
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Abstract Abstract: The parent strain Aspergillus niger LW-1 producing acidic β-mannanase was isolated naturally and then treated with vacuum microwave irradiation and EMS. A high- and stable-yield acidic β-mannanase producing strain Aspergillus niger E-30 was obtained through screened by solid-state fermentation on basis fermentation medium and many times of subcultures. Its enzyme activity was increased by 2.15 times from 17 048 U/g to 36 675 U/g. The E-30 properties of highly producing acidic β-mannanase remained stable after storage for two months.
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Received: 29 June 2007
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