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Abstract Lipopolysaccharide-binding protein(LBP) is key factor in identifying endotoxin of gram-negative bacterium and starting immune response. The aim of this study was to understand LBP gene codon use features and provide foundation for selecting appropriate receptor animals and expression systems. In this research, pig(Sus scrofa) LBP gene(GenBank Accession: NM-001128435.1) based on electronic cloning sequence was analyzed by Codon W, CHIPS, and CUSP programs online, and then compared with eight disease resistance-related genes, genomes in model organisms and LBP genes from 14 animal species. The results showed that most of pig LBP genes were bias toward the synonymous codons with G and C at the third codon position. There were twenty-seven biased codons (relative synonymous codon usage(RSCU)＞1), whose biased strongly codons were GCC, CAC, CTG and TCC (RSCU≥2). However, all of eight disease resistance-related genes were bias toward codons with G and C, and had twenty-three biased codons, whose biased strongly codons were GCC, ATC, CTG and GTG. The comparative analysis results of LBP genes from 14 animal species also showed that gene expression level was general in 14 animal species，and all of the LBP genes were bias toward the synonymous codons with G and C at the third codon position, which was possible due to special functions of these genes. Clustering analysis showed that pigs belonging to artiodactyla and animals belonging to carnivora were together for a class, which disagreed with taxonomic relationship. The differences in codon usage frequency between pig LBP gene and genome of Mus musculus were less than other two kinds of model organisms' (Escherichia coli and Saccharomyces Yeast) genome. Therefore, Mus musculus expression systems might be more suitable for expression of LBP gene. This study can provide certain theoretical basis for selecting the appropriate receptor animals in animal genetic improvement, selecting appropriate expression systems and improving the expression level of LBP gene.
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Received: 21 May 2013
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ZHU Xiao-Feng, XU Hou-Qiang, CHEN Wei, CHEN Ying-Lian, NI Kai, WU Xiao-Min, NI Meng-Meng, LU Xian-Jun. Cloning, Expression and Bioinformatics Analysis of IGF-1 and IGF-2 Genes in Congjiangxiang Pigs (Sus scrofa)[J]. 农业生物技术学报, 2019, 27(8): 1382-1391. |
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