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Abstract Abstract: To evaluate the effects of a system of serum-free maturation culture on in vitro maturation of goat oocytes and development of parthenogenesis embryos，0.3 % BSA (m/v), 1% PVA (m/v) and 1% ITS (v/v) were added to basic medium of oocyte maturation as substitute of serum, 5% fetal bovine serum was added to control. Cumulus-oocyte complexes (COCS) derived abattoir ovaries were cultured in vitro with those culture medium respectively. Maturation rate of PVA supplementation group (50.00%) was significant lower than that of control (83.23%)(P<0.01), and that of BSA group (71.19%)and ITS group(76.79%) had no difference compared to control; The matured oocytes were actived by ionoycin and 6-dimethylaminopurine, then, parthenogenesis embryos were cultured in SOFaa medium to observe development of embryos each group. Cleavage rate from BSA, PVA, ITS and FBS group was 61.90%, 46.97%, 79.07% and 83.58% respectively, and blastocyst rate respectively was 25.00%, 19.70%, 55.81% and 60.45%. The results show that substitute PVA and BSA for FBS in elemental maturation medium decrease significantly the abilities of maturation and early development of embryos(P<0.05 or P<0.01),substitute ITS for FBS decrease the abilities of maturation ,but has no effect on early development of embryos(P>0.05).Conclusion: ITS may be applied to in vitro maturation of goat oocyte as substitute of FBS.
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Received: 21 September 2006
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