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| Real-time Quantitative PCR Detection of Escherichia coli O157:H7 |
| CHEN Si;HUANG Kun-lun;XU Wen-tao;LI Yuan;LUO Yun-bo |
| College of Food Science, China Agricultural University, Beijing 100083, China |
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Abstract A rapid and accurate real-time quantitative polymerase chain reaction (Real-time PCR) method with SYBR GreenⅠfor detecting Escherichia coli O157:H7 was established. A pair of amplify primers were designed to amplify eae gene. The dissociation curves showed that the amplified product was very specific. The optimal reaction program and standard curve were founded. The result indicated that Real-time PCR was sensitive 1000 times more than ordinarily PCR.
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Received: 29 April 2005
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Corresponding Authors:
LUO Yun-bo
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