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| Cloning and Expression Analysis of the Up-stream Regulatory Sequence of Transcription Factor Gene BrWRKY33 from Chinese Cabbage |
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Abstract Abstract An 1 755 bp up-stream regulatory sequence of the transcription factor gene BrWRKY33 was cloned from Chinese cabbage (Brassica rapa subp. pekinensis cv. LongbaiⅡ). Two 5' deletion mutants of the sequence were fused with the reporter gene gus, respectively, and transformed into Arabidopsis thaliana. The results indicated that the W-box sequences present in －1 755~－315 bp region of BrWRKY33 was related to the down regulation, and the 0~－315 bp region contained the essential sequence for gene transcription. Transgenic plants challenged with soft rot bacteria (Erwinia carotovora subsp. carotovora) showed an increase in gus activity, indicating that BrWRKY33 gene play a role in the resistance against soft rot disease.
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Received: 07 April 2009
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