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Abstract ABSTRACT：The NP gene segment deleted part of sequence，with a length of 1320bp，and coding for 440 amino acids，was amplified by PCR．The NP gene fragment was then inserted into the EcoR I site of pR vector to obtain recombinant plasmid pR-NP. The recombinant plasmid was used to transform E．coli E2, the host bacteria of T4 phage. and then infected with lysozyme-defective phage T4-Z1.The NP gene were then integrated into the SOC site of the genome of SOC-deleted T4 phage mutant by homologous recombination. The recombinant phage were screened by PCR and designated as T4-ZI-NP．The immunological test applying Western blot showed that SOC-NP fusion protein expressed by the phage T4-ZI-NP could react to AIV specifically. Recombinant bacteriophage T4 expressing the NP Gene of Avian Influenza Virus was constructed successfully.
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Received: 20 November 2006
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