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Abstract Screening microsatellite marker from ESTs database (10691 item) of common carp, we have found 127 microsatellite sequences which occupied 1.19% of ESTs database. Besides these markers, we have isolated 20 microsatellite sequences from constructing cDNA library for common carp. These microsatellite sequences allowed us to design 68 pairs of primers with Primer Premier 5.0. 27 primer pairs obtained expected product, and 20 primers showed polymorphism among populations in synthesized 40 primers. Using the 20 polymorphic loci, we analyzed common carp genetic structure of two geography populations, Yangtze River and Heilongjiang River. The results showed that the average effect alleles numbers were 3.9135 and 3.4820 respectively; the average observed heterozygosity was 0.6067 and 0.5667; the average expected heterozygosity was 0.6737 and 0.6194; the average polymorphism information content was 0.6308 and 0.5714. Population structure of Yangtze River is better than that of Heilongjiang River. Hardy-Weinberg deviate index of the two populations were small, indicating that the populations was equilibrium. Amplified results in HLJE1 and HLJE10 were very different between Yangtze River and Heilongjiang River, and polymorphism in Yangtze River was much higher than in Heilongjiang River. The two loci may be used for common carp population identification of Yangtze River and Heilongjiang River.
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Received: 16 April 2007
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