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Influences of Nitrogen Ratio and Carbon Sources on Adventitious Shoots Regeneration of Malus zumi Leaves In Vitro
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| Li Bao Han Zhenhai** |
| (Institute for Horticultural Plants, China Agricultural University, Beijing 100094, China) |
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Abstract Abstracts: Factors that affect regeneration of adventitious shoots of Malus zumi Mats leaves in vitro were examined. The results showed that the changes of NAA concentrations were more responsive than that of BA; different ratios of NO3-:NH4+ in MS medium had different results, 2∶1 was the best, followed by 3∶1, 4∶1 and 1∶1; Fructose improved the regeneration of adventitious shoots significantly among the four tested carbon sources which were fructose, sucrose, glucose and sorbitol; And the optimimum medium was consisted of MS salt and vitamins and supplemented with BA (2.5 mg/L), NAA (0.25 mg/L), fructose (30 g/L), agar (8 g/L), and the pH was 5.8. The action mechanism of carbon sources was discussed in the paper.
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Received: 01 January 1900
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Corresponding Authors:
Han Zhenhai**
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