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Abstract BALB/c mice were immunized intraperitoneally with purified recombinant pET-mEIL-18 protein. Murine myeloma cells were fused with the splenocytes of the immunized mice after the third immunization. An indirect ELISA with recombinant mEIL-18 come from baculovirus expression system as antigen was used to screen antibody-producing hybridomas. 12 McAbs against pET-mEIL-18 were obtained. Nine hybridomas cells of them can produce McAbs steadily after 3 cycles of cloning and all of them showed positive reaction to mEIL-18 in IFA tests. The 9 McAbs were reactive with pET-mEIL-18-(1) or pET-mEIL-18-(2) respectively in specific ELISA tests. As a result, five McAbs of them showed positive reaction to pET-mEIL-18-(1) protein, others show positive reaction to pET-mEIL-18-(2) protein. Three McAbs are IgM isotype and the others belong to IgG1 isotype, the light chain of all McAbs is 僴chain. The McAbs against pET-mEIL-18 protein developed in the study would be useful as a basis of detection methods for mEIL-18, investigating the structure and biological effects of mEIL-18.
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Received: 14 February 2007
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