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Abstract High abundance and tissue-specific gene expression are important for gene therapy and preparation of transgenic animals. Cre-LoxP system is effective to improve transcriptional activity of tissue-specific promoter. However, it is not known if the Cre-LoxP system affects the tissue-specific expression regulated by the specific promoter.. In this study, intestinal mucin 2 promoter was used to regulate the Cre recombinase expression base on Cre-LoxP system. Then luciferase activity was detected after transfection. The results showed that Cre-LoxP system increased the expression of mucin 2 promoter -mediated target genes (6-fold) in intestinal cells. However, cell-specific tests showed that the system decreased the specificity of mucin 2 promoter. The results suggest that the use of Cre-LoxP system requires higher specificity of the promoter although the system can significantly improve the weak promoter activity.
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Received: 27 April 2011
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HUANG Xing, YAN Ai-Fen, DENG Ting-Xian, OUYANG Hong-Jia, LIU Lian, FENG Juan, ZHU Xiang-Xing, NIE Qing-Hua, TANG Dong-Sheng, ZHANG Xi-Quan. Construction of Zinc Finger Nuclease-induced Targeting Vector of Luchuan Pig (Sus scrofa) Fat1 Gene and Transgenic Study In vitro[J]. 农业生物技术学报, 2019, 27(8): 1369-1381. |
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