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Abstract To identify grape (Vitis vinifera L.) genes in response to exogenous gibberellins (GA) treatment, a large number of grape EST sequences collected at NCBI were analyzed. Forty-five non-redundant EST sequences that only expressed after GA treatment were obtained by using local Blast, Blast2go and other programs. Blastx annotation results indicated that twenty-five sequences(55.6%) were of hypothetical or unknown protein, fourteen sequences(31.1%) did not have available information, and six sequences(13.1%) had predict functions. The six sequences with annotated functions carried genes encoding integrase (EE077049), SPX domain (EE085000), serine carboxypeptidase-like 44(EE091188), CHY1 (EE092187), pseudouridine synthase/ transporter (EE106096) and K+ channel protein (EE108944). Blast2go analysis showed that only twenty-nine sequences (64.4%) had available Gene Ontology (GO) annotations, belonging to categories of molecular function, cellular component or biological process. Taken together, the GA-responsive gene products mainly had binding activities (46.34%) or catalytic activities (39.02%), distributed in the whole cell (44.68%) or in specific organelles (40.43%), participated1 in cellular processes (28.57%) or metabolic processes (25.71%) in responding to the exogenous GA treatment. This study provides basic information for further analysis of gene expression in response to exogenous GA treatment.
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Received: 20 June 2011
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