Contact Us Add to Favorite
 
NianQi Search Adv Search
33
研究报告 Current Issue | Archive | Adv Search |
Gene Expression Analysis of Key Enzymes and MYB Transcription Factors in Flavonoid Biosynthesis Pathway during Germination of Fagopyrum tataricum
Download: PDF (651 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  As a medicinal and edible plant, tartary buckwheat (Fagopyrum tataricum) is rich in flavonoids especially rutin. During germination, it has a higher concentration of ruin, but the molecular mechanism is still unclear. In this study, the total flavonoids in cotyledon and hypocotyl of F. tataricum during 6~10 days after germination were extracted by the methanol extraction method, and were determined by aluminum chloride spectrophotometry. Semi-quantitative RT-PCR was used to determine the expression level of the phenylalnine ammonialyase gene (Pal), chalcone isomeerase gene (Chi), flavonol synthase gene (Fls) and MYB transcription factor genes (FtMyb1, FtMyb2 and FtMyb3) in the cotyledon and hypocotyl during the seed germination, the relation among them analysed by statistics method. The result showed that: in cotyledons, the total flavonoids content was significantly and positively associated with FtMyb3 (0.9625), and negatively with FtMyb2 (-0.8572); Chi was significantly and positively associated with FtMyb2 (0.8468), and negatively with FtMyb3 (-0.8010); the three key enzyme genes were significantly and positively correlated to each other with 0.9119, 0.8920 and 0.7584, respectively. In hypocotyls, the total flavonoids content was significantly and negatively associated with Chi (-0.8989), while Fls was significantly negative associated with Chi (-0.7498). Visiblely, the regulation of flavonoid biosynthesis is rather complex during germination of F. tataricum, but some gene's expression is still significantly associated with each other.
Key wordsFagopyrum tataricum      germination      total flavonoids content      key enzyme gene      MYB transcription factors      Semi-quantitative Polymerase Chain Reaction     
Received: 19 August 2011     
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
NULL
Cite this article:   
NULL. Gene Expression Analysis of Key Enzymes and MYB Transcription Factors in Flavonoid Biosynthesis Pathway during Germination of Fagopyrum tataricum[J]. , 2012, 20(2): 121-128.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2012/V20/I2/121
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech