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| Cloning of Bovine Interleukin-1 Receptor-associated Kinase 2(IRAK2) Gene and Its Bio-information Analysis |
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Abstract Toll-like receptors (TLRs) can recognize the pathogen associated molecular pattern and mediate signaling for initiate innate and adaptive immune responses. Interleukin-1 receptor-associated kinase 2 (IRAK2) is one of the important downstream signal molecules in TLRs signal transduction pathways. In order to further study the function and signal transduction of TLRs on the diseases induced by pathogen infection, bovine IRAK2 gene isolated using RT-PCR and RACE experiments was analyzed by bio-informatics and tissues expression profiles. The results showed that bovine IRAK2 gene could be expressed in many tissues including mammary gland, liver, duodenum, fat, womb, kidney, heart, lung, pancreas and ovary. Its cDNA sequence was 2 148 bp (GenBank accession No. EU528620), including 36 bp 5'-UTR, 1 866 bp CDS and 246 bp 3'-UTR. IRAK2 gene codes 622 aa, molecular weight and isoelectric point are 68 628.31 D and 5.3, respectively. IRAK2 protein contained the death domain(DD) and S_TKc domain and located at lots of cell organs such as cell nucleus, cytoplasm, mitochondrium and protoplasmic membrane. It is inferred that IRAK2 may be very important for signaling and regulating on TLRs signal transduction.
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Received: 02 June 2008
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