Abstract A series of comparative test analysis were conducted and results showed that the effect of detection of RNA by gel electrophoresis was not only associated with comb size and RNA concentration, but also associated with migration time in gel electrophoresis and the amount of RNA to be loaded. Finally, a facile, rapid and effective method for RNA quality determination was established, namely using 1.2% agarose gel with the comb of breadth 7 mm, loaded 3~5 μg RNA, mixed with 6×RNA loading buffer and diluted with RNase-free water, followed by electrophoresis for 15~30 min and the reality of RNA quality could be determined. This method had the characteristic of reduplicate in practice, and can be used as a good reference for researcher.
