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| Expression and Identification of Peste des petits ruminants virus N Gene in Escherichia coli |
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Abstract A pair of primers added EcoRⅠand NotⅠsites was designed and synthesized based on the sequence of the nucleocapsid（N）protein gene of Peste des petits ruminants virus (PPRV), which was for amplifying the full-length N gene fragment of pCI-neo -PPRN plasmid from Austria. The fragment was cloned into pGM-T vector and transformed into Escherichia coli TOP10. Then the cloned fragment was subcloned into prokaryotic expression vector pET-32a(+), and the recombinant expression plasmid pET-PPRN was expressed in E.coli BL21 (DE3) with inducement. The size of the recombinant N fusion protein was 80 kD. The recombinant PPRV N fusion protein could be detected with Histidine monoclonal antibody or goat anti-PPRV sera by Western blot.
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Received: 16 May 2008
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