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| Cloning and Analysis of the Homologous Fragments of BADH Gene from Several Compositae Plants |
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Abstract BADH gene was tested in 26 species of Compositae and 2 chrysanthemum cultivars by PCR-Southern. 18 species and 2 cultivars showed clear positive signal. The PCR products of 7 species and 1 cultivar were sequenced（GenBank accession No. EF683587-683595）. 2 homologous fragments of BADH gene , which were very different in length, were obtained from Inula japonica. Sequence analysis showed that the cloned exon regions were identical to each other in length, the homology among them was above 83% , and the homology among the deduced amino acid sequence was above 90%. The second site changed from threonine to alanine in the conserved decapeptide in the longer fragment from Innula japonica. The length of intron region was very different among the cloned sequences. There was not apparent homogology among the intron regions from Eupatorium lindleyanum, Stemmacantha uniflora and the intron region of the longer sequence from Inula japonica. And there was not apparent homology between the 3 intron regions and any other intron regions. However, there was apparent homology among the other intron regions. And the dendrogram of gene relationships from the cloned intron sequences was almost identical to that from exon sequences.
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Received: 22 October 2007
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