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| Genetic Polymorphism of OPN Gene and Correlation with Reproductive Traits in Pig |
| Zhuo-Ma LUOREN Li-xian WANG Shi-duo SUN |
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Abstract Abstract:Osteopontin gene was regard as a candidate gene for reproductive traits of pig in recent years, in this study, genetic polymorphism of OPN gene and correlation with reproductive traits was studied in Landrace pig, Large White pig and Beijing Black Pig. The polymorphism was found by the PCR, which was induced by a deletion mutation of 305bp in the sixth intron of OPN gene after sequencing. AA, AB and BB genotypes were found in Landrace pig and Large White pig populations, which showed a moderate polymorphism. The statistical results of χ2-test indicated that this locus fitted Hardy-Weinberg equilibrium. While polymorphism of this locus was not found in Beijing Black pig, and the BB genotype was only present. Meanwhile, the effect of polymorphism for OPN gene on total number born (TNB), number born alive (NBA) and born weight (WB) were analyzed, the results indicated that the individuals of the first parity Landrace pig with AA genotype showed more TNB and NBA significantly than that with AB and BB genotype (P<0.05), the individual of multiparous sows with AB genotype showed more TNB and NBA significantly than that with AA and BB genotype (P<0.05), and A allele might have the positive effective on TNB, NBA and WB. While in Large White pig, the TNB and NBA of sow with BB and AB genotype were more than those with genotype AA, but the results showed no significant difference (P>0.05), and B allele might have the positive effective on TNB and NBA.
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Received: 27 August 2007
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Corresponding Authors:
Li-xian WANG
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