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| Polymorphism of MC4R Asp298Asn Site and its Relationship with Backfat Thickness in Commercial Pigs |
| Yun-Liang JIANG Xiao－Hui YANG Yuan LIU Ning－Bo ZHANG Ying WU |
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Abstract Melanocortin- 4 receptor (MC4R) is a member of the superfamily of G protein coupled receptors (GPCRs), which affects body weight, energy homeostasis and food intake in human and mouse. In this study, the Asp298Asn polymorphism of MC4R gene in Laiwu, Yorkshire×Laiwu and commercial crossbred pig populations was investigated using PCR-RFLP method, and, the relationship of this mutation with backfat thickness was analyzed. The results indicated that only genotype 11 exist in 33 individuals of Laiwu pigs, whereas in Yorkshire×Laiwu and commercial crossbred populations, three genotypes of 11, 12 and 22 were detected. The distributions of allele and genotype frequencies in Yorkshire×Laiwu and commercial crossbred populations were similar with the frequency of allele 1 being higher than that of allele 2. All of the three populations were at Hardy-Weinberg equilibrium with respect to this mutation site（P>0.05）. In commercial crossbred pigs, individuals with genotype 22 had significant higher backfat thickness than the ones with genotypes 12 and 11 (P < 0.05). This study provide evidence that the Asp298Asn polymorphism of MC4R is associated with backfat thickness in commercial crossbred pigs with western pigs as parental lines, and therefore, can be used as a DNA marker for pig breeding in such kind of populations.
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Received: 08 October 2007
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Corresponding Authors:
Yun-Liang JIANG
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ZHU Xiao-Feng, XU Hou-Qiang, CHEN Wei, CHEN Ying-Lian, NI Kai, WU Xiao-Min, NI Meng-Meng, LU Xian-Jun. Cloning, Expression and Bioinformatics Analysis of IGF-1 and IGF-2 Genes in Congjiangxiang Pigs (Sus scrofa)[J]. 农业生物技术学报, 2019, 27(8): 1382-1391. |
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