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| The histological characteristics of longissimus dorsi and its correlation with Msp I polymorphism of calpastatin and myogenin gene in F2 Jinghua×Pietrain crossbred pigs |
| Jiu-Sheng Wu Ning-Ying Xu |
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Abstract In order to evaluate the genotype of CAST gene and its relationship with the muscle histology and other postmortem traits in Jinhua×Pietrain F2 pig family. 158 Jinpi F2 pigs were electrically stunned and exsanguinated. The blood and muscle samples were collected, and both postmortem and meat traits were recorded. PCR-RFLP, PAS and myosin heavy chain immunohistochemistry were employed to explore the relationship between the genotypes and the muscle histology. Myosin heavy chain immunohistochemistry could differentiated the fibers into slow fibers and fast fibers, the ratio of slow fibers is about 7.62:92.38%. The amplification products of the region between 6th and 7th exon of CAST gene were digested by MspI and discriminated 2 polymorphic sites(371bp and 628bp) in endonuclease map. Only 3 genotypes (AA, AB and BB) was distinguished out. The frequency of AA,AB and BB is 0.1795, 0.5897 and 0.2308 respectively. The frequency of A and B is 0.4743 and 0.5256 respectively. The PCR products of the MyoG gene were digested with MspI and 2 polymorphic sites(290bp and 462bp) were distinguished. The allele C was defined as 408+290+180bp, the allele D was defined as 462+408+180bp and 3 genotypes (CC, CD and DD) were discriminated. The frequency of CC,CD and DD is 0.3671,0.5696 and 0.06330 respectively. The frequency of C and D is 0.6519 and 0.3481 respectively. Different genotypes of these tow gene have no statistical significant effect on area, water holding capacity, pH45m value, conductivity of loin muscle. The results revealed that the MspI polymorphism of CAST gene had a significant effect on the aspect ratio of muscle and show a negative correlation with the percentage of intramuscular connective tissue. The MspI polymorphism of MyoG gene had a significant effect on the fiber size and fiber density. The individual with DD genotype had more big-sized fibers and the individuals with CC genotype had high fiber density.
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Received: 06 July 2007
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Corresponding Authors:
Jiu-Sheng Wu
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NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
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