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Abstract Perilipin gene(PLIN) plays a crucial role during lipid metabolism and fat deposition. In order to reveal the genetic effects of polymorphisms in PLIN on carcass and fatness traits in chicken, PLIN gene polymorphisms in 384 Luqin chicken (Gallus gallus)were detected by DNA sequencing and PCR-RFLP methods. Three single nucleotide polymorphism (SNP) loci, g.2272C＞T, g.2319C＞T and g.2467G＞A in PLIN gene were found, and their genetic effects on carcass and fatness traits were analyzed by sas6.12. Based on the 3 SNPs, 7 haplotypes were constructed by PHASE2.0. C-C-G was the major haplotype with the frequency of 71.94%, while the frequencies of C-C-A and C-T-A were lower than 1%. Eleven diplotypes were obtained from the 7 haplotypes, H1H1 was the dominant diplotype with the frequency of 52.60%. Statistical analysis showed that diplotype was extremely significantly associated with living body weight, carcass weight and leg muscle weight (P＜0.01), and significantly associated with abdominal fat weight and percentage of abdominal fat (P＜0.05), as well as breast intramuscular fat (P=0.094 9). The multiple-range test indicated that H1H3 chickens had the highest living body weight, carcass weight, leg muscle weight, abdominal fat weight and percentage of abdominal fat, followed by H4H6 chickens. H6H6 chickens were the lowest. The breast intramuscular fat content was the highest in H4H6 chickens and the lowest in H1H3 chickens. The results indicated that H6H6 was disadvantaged for chicken carcass and fatness traits, which should be removed during the breeding, while H4H6 was an advantaged diplotype which was suggested to be chosen to improve the carcass traits and meat quality of Luqin chickens. The research build the theoretical basis for the PLIN gene applied in poultry molecular breeding.
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Received: 21 March 2014
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