Abstract In order to study the mechanism of flue-cured tobacco aroma style formation that the same tobacco variety in different ecological area, we extracted chloroplast from tobacco (Nicotiana tabacum) leaves which were planted in the typical delicate aroma style ecological region Yuxi and the typical strong aroma style ecological region Guiyang, then used TCA-acetone method to extract chloroplast protein, by using two-dimensional electrophoresis combined with mass spectrometry to analysis chloroplast differential proteomics of typical strong aroma style and delicate aroma style tobacco leaves. Between the molecular weight 14.3~97.2 kD, in Guiyang the detectable protein spots was 1 270, of which the increased expression protein spots were 41, the specific expression protein spots were 10; in Yuxi the detectable protein spots were 892, of which the increased expression protein spots were 48, the specific expression protein spots were 4, the co-expression protein spots were 716. Using MALDI-TOF-TOF mass spectrometry and bioinformatics to compare the specific expression14 protein spots, Guiyang had 6 homologous matching protein, ATP synthase subunit beta, Rubisco and NADPH dehydrogenase; Yuxi had 1 homologous matching protein, plastidic aldolase NPALDP1. The closely related to the photosynthesis of protein specificity expressed in Hunan Guiyang; The closely related to glucose metabolism of protein specificity expressed in Yunnan Yuxi. To our knowledge，this is the first attempt to explore the mechanism of tobacco aroma style formation on a chloroplast proteome level, providing a theoretical basis for the breeding and production of Flue-cured tobacco.
