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| Application of Real-time Fluorescence Quantitative PCR Technology for Porcine Growth Hormone Gene Differential Expression of Transgenic Pigs |
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Abstract In order to confirm genetic stability and early fattening expression level difference of foreign porcine growth hormone gene(pGH) in F1 generation of transgenic pigs, growth hormone in 7 trans-pGH gene pigs and 9 non-transgenic pigs of 3-month-old were detected by the advantage of SYBR GreenⅠReal-time PCR method, which were produced by F0 transgenic sows made by nano-gene carrier method induced by sperm and normal boar. According to amplification curve and melting curve maps, Ct mean value was gained through the equation F=(1+E)-ΔΔCt. The results showed that pGH average expression level in F1 generation of transgenic pigs was 1.77 times as non-transgenic pigs. Suggesting that: 1) foreign gene pGH could stably passage in transgenic pigs, 2) not every pGH gene's expression level in transgenic pig was higher than that of non-transgenic pig, 3) average expression level of pGH gene in F1 generation transgenic pigs was observably higher than that of non-transgenic pigs in the same period. A set of molecule biology method of evaluating pGH relative expression level stably was built, and provided scientific method for discussing physical signs and quantitative expression between transgenic and non-transgenic animals
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Received: 27 March 2012
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HUANG Xing, YAN Ai-Fen, DENG Ting-Xian, OUYANG Hong-Jia, LIU Lian, FENG Juan, ZHU Xiang-Xing, NIE Qing-Hua, TANG Dong-Sheng, ZHANG Xi-Quan. Construction of Zinc Finger Nuclease-induced Targeting Vector of Luchuan Pig (Sus scrofa) Fat1 Gene and Transgenic Study In vitro[J]. 农业生物技术学报, 2019, 27(8): 1369-1381. |
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