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| Molecular Characterization of Tomato yellow leaf curl virus(TYLCD) Infecting Tomato (Lycopersicon esculentum) in Jingyang County of Shaanxi Province |
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Abstract A total of 73 tomato(Lycopersicon esculentum) samples exhibiting stunting, yellowing and leaf-curling symptoms of tomato yellow leaf curl disease (TYLCD) were collected in Jingyang, Shaanxi Province. Using degenerate primer pair PA and PB of geminiviruses and specific primer pair TYT-F and TYT-R of Tomato yellow leaf curl virus (TYLCV), geminiviruses and TYLCV were detected in 70 samples by PCR. The DNA-B and satellite DNA molecules of geminiviruses were not detectable from all these samples with their universal primers respectively. Using rolling circle amplification PCR (RCA-PCR), cloning and sequencing techniques, the complete nucleotide sequence of isolate SX8(GenBank Accession No. JN412854)was determined to be 2 781 bp and has highly identity of nucleotide sequences (99.9%) with TYLCV-[SD2] (GenBank Accession No. GU199587)isolated from tomato in Shandong Province. The phylogenetic analysis indicated that TYLCV-[SX8] shared high relativity with those previously reported isolates of TYLCV in China. These results suggest that TYLCD in Shaanxi Province is caused by TYLCV which may originate from DNA-A molecules of TYLCV infecting tomato in Shandong Province.
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Received: 08 June 2012
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