|
|
|
Abstract Polyamines are ubiquitous organic cations of low molecular weight. The role of polyamines in the animal digestion, nutrient metabolism and normal growth is considered. We cloned polyamine metabolism-related genes polyamine-modulated factor 1(PMF1), arginine decarboxylase(ADC), ornithine decarboxylase(ODC), ornithine aminotransferase(OAT), ornithine decarboxylase antizyme 1~3(OAZ1, OAZ2 and OAZ3), their fragment length were 1 602(GenBank No. EU545649), 1 422(GenBank No. EU534186), 1 465(GenBank No. EU404089), 874(GenBank No. EU545195), 678(GenBank No. EU545196), 667(GenBank No. EU545197) and 703 bp(GenBank No. EU534185), respectively, with the open reading frame(ORF) of 1 380, 1 383, 1 313, 681, 568, 559 and 615 bp and, coding amino acids of 460, 461, 439, 227, 189, 186 and 205, respectively. The results of comparative analysis illustrated that the porcine ADC encoding of amino acid sequence shared 89.7% and 87.4% similar to that of human (Homo sapiens) and mouse(Mus musculus) respectively; the porcine ODC one shared 93.1% , 89.8% and 90.5% similar to that of human, mouse and rat respectively; the porcine OAT one shared 91.8%, 90.5% and 89.8% similar to that of human, mouse and rat (Rattus norvegicus) respectively; the porcine OAZ1 one shared 92.5%, 85.1% and 84.3% similar to that of human, mouse and rat respectively; the porcine OAZ2 one shared 98.1%, 97.9% and 97.2% similar to that of human, mouse and rat respectively; the porcine OAZ3 one shared 90.4%, 87.5% and 84.2% similar to that of human, mouse and rat respectively; the porcine PMF1 one shared 84% and 70.9% similar to that of human and mouse respectively. The analysis of the semi-quantitative RT-PCR showed that, after weaning, ADC mRNA increased significantly in appendix and rectal, while decreased slightly in other tissues; ODC mRNA increased significantly in appendix and rectal, while decreased slightly in jejunum, ileum and colon; OAT mRNA increased significantly in renal, while decreased slightly in duodenum, jejunum and ileum; OAZ1 mRNA increased in different magnitude, except in stomach; OAZ2 mRNA increased in different magnitude, especially in the appendix; OAZ3 mRNA increased in different magnitude in the intestinal tissues; PMF1 mRNA was similar to the (AZ) family in jejunum, ileum and colonic, but decreased slightly. These results suggest that polyamine are involved in the effect of the growth, development, mature, adaptability and repair of injured in piglet
|
|
Received: 27 March 2012
|
|
|
|
|
|
