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Abstract Iron (Fe) is important and indispensable element for the normal physiological processes in the human body, and iron deficiency is a serious nutritional problem in the world, so it is economical and effective to breed and grow wheat cultivars with high grain iron content, especially for those population in poor-developed areas. In this paper, the soybean (Glycine max) ferritin gene was obtained by RT-PCR, and cloned into the pBAC47P vector. The soybean ferritin expression cassette without vector backbone sequence but including wheat endosperm-specific HMW-GS 1Dx5 promoter and NOS terminator was constructed by digestion and purification, and has been transformed through particle bombardment of PDS1000/He system into winter wheat(Triticum aestivum) cultivars, Jinghua No.1. After phosphinothricin (PPT) selection, differentiation and regeneration, 276 plants were regenerated from immature embryos callus. By PCR screening and PCR-Southern blot using specific primers for soybean ferritin gene 65 transgenic plants of T0 generation were selected, Among them, kernels of 29 plants of T1 generation were positive by RT-PCR on the RNA levels. The grain iron content of 8031 T1 transgenic lines have been determined. Compared to the control, there were 265 lines of them have increased iron content, ranging from 4.93 to 64.03 percent, and 22 lines of them increased significantly, which suggests that Fe content of wheat grain can be improved by the soybean ferritin expression cassette without vector backbone sequence
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Received: 22 December 2011
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Corresponding Authors:
Rong-Qi LIANG
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