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Abstract In the past two decades, many countries have been paying more attention to safety of genetically modified (GM) product, and thus, qualitative and quantitative test of GM products become more and more important. The test result can be more comparable and reliable by using GM reference material. This paper reviews the research progress of GM reference material and related techniques: it introduces the variety of GM reference material and techniques of value assignment for GM reference material, and also evaluates the key procedures in developing GM reference material. More importantly, it is concluded that how to assign the reference value for the plasmid reference material including the commutability between the plasmid and genomic DNA. Additionally, it summarizes the current international GM certified reference material. This aims to provide some valuable reference for developing GM reference material in China.
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Received: 02 September 2011
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HUANG Xing, YAN Ai-Fen, DENG Ting-Xian, OUYANG Hong-Jia, LIU Lian, FENG Juan, ZHU Xiang-Xing, NIE Qing-Hua, TANG Dong-Sheng, ZHANG Xi-Quan. Construction of Zinc Finger Nuclease-induced Targeting Vector of Luchuan Pig (Sus scrofa) Fat1 Gene and Transgenic Study In vitro[J]. 农业生物技术学报, 2019, 27(8): 1369-1381. |
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