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Abstract Abstract: One novel vegetative insecticidal protein (vip) gene, vip3A-LS1, was cloned and identified as GenBank accession number DQ016968 (Vip3Aa22). Alignment results showed that the Vip3A-LS1 deduced protein had 8 amino acids that were different from published homologous proteins. Vip3A-LS1 inserted into pQE30 and transformed into Escherichia coli M15 was expressed to a high level after being induced by IPTG. The intracellular soluble protein extracted from the transformant possessed high toxicity to the larvae of Helicoverpa armigera and Spodoptera exigua, and the LC50 of its purified protein was 73.6 ug/g to H. armigera, and 32.2 ug/g to S. exigua respectively.
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Received: 29 January 2007
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