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| Construction of expression vector for enhancing the expression of the Psy gene and restraining the expression of Lcy gene |
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Abstract Lycopene is a kind of carotenoid. Because of its important role in preventing some diseases such as the cancer and the heart disease the lycopene is becoming the research hotspot as the function food in recent years in the world. Increasing the express of Psy gene and blocking the express of Lcy gene which is the main gene regulating the transformation of the lycopene is the convenient way to enhance the content of lycopene. The primers were designed according to the gene sequence（NM-121729）、（U46919）and (X86452) in GenBank. The Psy gene was isolated from the cDNA of the Arabidopsis thaliana. The Pds promoter and the DNA segments of the Lcy gene were obtained from the genome DNA of tomato. The 3’end of lcy DNA segment was connected together by an intron to inform the RNA interferential segment then which was inserted in the expression vector with the fruit-specific promoter of phytoene desaturase gene(pds)；The Psy gene was inserted in the same vector with the Pds promoter. Then the expression vector was obtained which can increase the expression of Psy gene and interfere the expression of Lcy gene at the same time by transformant into the plant with the carotenoid.
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Received: 19 September 2006
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