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| Development and evaluation of the near-isogenic lines for QTLs of basal root thickness and 1000-grain-weight in lowland and upland rice |
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Abstract The development of near-isogenic lines (NILs) is one of the important foundations for the establishment of molecular linkage map, the mapping of quantitative trait loci (QTL) and molecular marker-assisted selecton (MAS). In the present study, NILs for two major QTLs of basal root thickness(BRT) and 1000-grain-weight(TGW) were obtained through the foreground selection for target QTL, the background selection for the genome of the recurrent parent of 3 backcross populations( BC1F1、BC2F1、BC3F1) and phenotypic selection for the population of BC3F2. They were 9 of BRT QTL-NILs with BRT ranges of 1.07~1.16mm, over the recurrent parent by 6.11%~15.18% and an average recovering ratio of genetic background (RRGB)of 97.22%; 11 NILs of 1000-grain-weight (TGW) QTL with ranges of 21.25～26.25g, over the recurrent parent by 7.05％～32.16％ and 95.97% of RRGB. The number of molecular markers for background selection in the development of NILs and QTL cloning were also discussed.
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Received: 27 September 2006
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