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Abstract Abstract: Purified PM-VP2-E290 and Bac-N-Blue DNA were cotransinfected insect cell sf9. The recombinant baculovirus were selected by their lacZ phenotypes and plaque-purified until no more wild type virus could be detected. Then, high-titer viral stocks of the recombinant baculovirus were prepared and used for expression. After SDS-PAGE analysis, an extra band of 67KDa was observed in the extracts of infected sf9 insect cells.The chimeric proteins had strong positive reactions with PPV specific antibody and E290-specific antibody. A large number of virus-like particles formed by expressed protein were observed under Immuno-electron microscopy (IEM).The BALB/c mice injected with the expressed protein, in the absence of adjuvant. showed that the hybrid recombinant parvovirus like-particles formed by the self-assembly of the VP2 capsid protein of PPV carring the CSFV epitope at its N terminal, not only induced a strong CD8+ class restricted CTL response, But stimulate a highly PPV specific antibody levels. Moreover, the antibody levels were significantly higher than that of the inactivated vaccine control.
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Received: 14 February 2007
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