|
|
|
| PCR-SSCP Analysis on Ghrelin gene in yellow chicken of two breeds |
| |
| |
|
|
|
|
Abstract Abstract： Two primers are designed on the base of Ghrelin gene sequence in genbank in this study, and the polymorphism of Ghrelin gene in 140 Jinghai yellow chickens and 30 Suqin yellow chickens was analyzed by PCR-SSCP. The result indicates that there were no polymorphism detected by Primer 1,and there were polymorphisms detected by Primer 2. BB, BC and CC genotypes were detected in Jinghai yellow chicken ,but only BB and BC genotypes were detected in Suqin yellow chicken; the allele B was obviously higher than that of C allele in two chicken breeds. The frequency of BB genotype is 0.933 in Suqin yellow chicken and 0.657 in Jinghai yellow chicken. The sequencing results showed that there was one single nucleotide mutation : T→A at the point of 546bp of Ghrelin gene in chicken.
|
|
Received: 27 November 2006
|
|
|
|
|
|
|
