Contact Us Add to Favorite
 
NianQi Search Adv Search
33
研究论文 Current Issue | Archive | Adv Search |
Expression and activity assay of recombinant bovine interleukin-18 in E.coli
Download: PDF (0 KB)  HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  To express the bovine interleukin-18(BoIL-18) protein, cDNA fragment encoding the mature BoIL-18 protein was subcloned into pGEX6p-1 and transformed into E.coli BL21(DE3) . The expressed BoIL-18 protein was purified by affinity Chromatography. MTT method ,ELISA method and VSV were used to assay the functional activity of BoIL-18 protein. Results: The recombinant BoIL-18 was highly expressed in E.coli and purified by affinity Chromatography method. The reBoIL-18 could enhance the proliferation of PBMC, induce IFN-γproducing in spleen lymphocyte. Conclusion: The purified reBoIL-18 has functional activity and can be applied for further studies.
Key wordsBovine Interleukin-18      prokaryotic Expression      Purification      Activity assay     
Received: 24 October 2006     
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Cite this article:   
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2007/V15/I4/0
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech