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Abstract Abstract: To study immunoprotection against Schistosoma japonicum(Sj) elicited by immunization of DNA vaccine co-encoding SjFerritin and GM-CSF. Methods SjFer were amplified by PCR from pGEX-5x-3/SjFer temple. GM-CSF were amplified by RT-PCR from totle mice RNA. Reconstructed plasmids were constructed by routine methods. Eighty-five female mice were randomly divided into 5 groups, 17 each .Group A, B and C are controls, immunized with monmal saline, pcDNA3.0 and GM-CSF/pc respectively, and group D, E were immunized with SjFer/pc and GM-CSF-SjFer/pc at weeks 0,2,4. Sera were collected before immunization and challenge infection . Serum lgG were detected by ELISA, Spleen lymphocyte cells of mice were collected before challenge infection.Proliferation activity of spleen lymphocytes was detected by MTT assay. Mice were infected with (40±1) Sj cercariae per mouse 2 weeks after final immunization. Forty-five days later, adult worms and eggs were counted. Results Level of IgG in sera increased after immunization. And proliferation activity of spleen lymphocytes were increased compared with group A, B and C. The worm reduction rate of groups D and E were 36.27 % and 39.22 %. The egg reduction rate of them were 36.10 % and 49.04 %, respectively. Inclusion GM-CSF increased immunoprotection against challenge infection of Sj.
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Received: 21 November 2006
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