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| Cloning and characterization analysis of spike protein gene of porcine Coronavirus |
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Abstract Abstract: The spike protein gene of porcine epidemic diarrhea coronavirus (PEDV) DX strain was cloned and sequenced. The results showed that the nucleotide sequence of the gene was 4152 nt in length and encoding a protein of 1383 aa, the homology of amino acids between it and other PEDV strains exceeded 90%. The results of phylogenetic tree analysis showed all the reference PEDV isolates could be divided into 3 genetic groups and the isolates in the same group came from the same country. Bio-soft analysis showed that PEDV muting slowly, especially in Korea, DX strain and JS-2004-2 strain have a closed relationship.
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Received: 18 December 2006
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