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| Cloning and expression of β-mannanase gene from Bacillus subtilis |
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Abstract A selected strain of Bacillus subtilis A33 which can hydrolysis the glucomannan of Amorphophallus is used as material to isolate the full-length gene of β-mannanase by PCR. Sequence analysis show this DNA fragment encodes β-mannanase which belongs to the family of mannanase.This Sequence had was submitted in GenBank（GenBank Accession：DQ269473）.To express it in E. coli it is recombinant into the high efficiency expressive vector pET-32a and a recombinant plasmid pET-32a-ManA is constructed and transformed into strain E. coli BL21（DE3）.β-mannanase is expressed efficiently after inducing.
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Received: 05 June 2006
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