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| Cloning and characterization of outer membrane protein H of toxigenic Pasteurella multocida strain HN-13 of swine |
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Abstract Progressive atrophic rhinitis is an important respiratory disease in swine industry, caused by toxigenic Pasteurella multocida. The OmpH of Pasteurella mumtocida is a major outer membrane protein involved in the pathogenesis and immunity. In this study the ompH gene of P. multocida strain HN-13 of swine origin was cloned, sequenced and highly expressed in E. coli, and an indirect enzyme-linked immunosorbent assay (ELISA) was established using the recombinant protein. The structure of the ompH gene and its protein was analyzed using bioinformatics methods, suggesting that the OmpH is a porin.
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Received: 12 July 2006
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