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Cloning and characterization of outer membrane protein H of toxigenic Pasteurella multocida strain HN-13 of swine
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Abstract  Progressive atrophic rhinitis is an important respiratory disease in swine industry, caused by toxigenic Pasteurella multocida. The OmpH of Pasteurella mumtocida is a major outer membrane protein involved in the pathogenesis and immunity. In this study the ompH gene of P. multocida strain HN-13 of swine origin was cloned, sequenced and highly expressed in E. coli, and an indirect enzyme-linked immunosorbent assay (ELISA) was established using the recombinant protein. The structure of the ompH gene and its protein was analyzed using bioinformatics methods, suggesting that the OmpH is a porin.
Key wordsPasteurella multocida      outer membrane protein H      cloning      expression      structural prediction      ELISA     
Received: 12 July 2006     
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http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2007/V15/I2/0
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