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Abstract Fe(II) transporter gene MxIrt1 was cloned from Malus xiaojinensis Cheng et Jiang. MxIrt1 has the conserved domain of ZIP gene family. It was supposed to be involved in the iron absorption. Full length of MxIrt1 gene under the regulation of 35S promoter was introduced into Arabidopsis mutant irt1-1 by Agrobacterium tumefaciens. GUS staining and Southern analyses indicated that MxIrt1 gene has been integrated into Arabidopsis genome. The transgenic Arabidopsis plants showed increased tolerance to iron stress than the mutant.
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Received: 20 April 2006
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