|
|
|
Abstract The AFLP markers was applied to analyze the genetic structures of Oreochromis niloticus, Oreochromis aureus and their hybrids F1, as well as the orange-colored Oreochromis mossambicus, Oreochromis hornorum and their hybrids F1. 8 pairs of primers
had been chosen from ninety pairs of primer combinations for selection amplification. A total of 250 loci had been produced using these 8 pairs of primers (100-500bp in length for each segment). The number of the amplified bands detected by each AFLP primer combination for each individual ranged from 20 to 47. The analysis indicated that the intraspecies genetic similarity of the four tilapia, arranged from high to low, were Oreochromis aureus (0.913), O. niloticus (0.871), O. hornorum (0.829) and O. mossambicus(0.784). The genetic similarity and genetic distance between O. niloticus and Oreochromis aureus were 0.811 and 0.189 respectively, while the genetic distance of the parent fish, O. niloticus, O. aureus and their hybrids F1 (Ni-ao) were 0.165 and 0.142 respectively. The genetic similarity and genetic distance between O. mossambicus and O. hornorum were 0.756 and 0.244 respectively, while the genetic distance between the parents and their hybrids F1 (Mo-he) were 0.148 and 0.162 respectively. Specific segments were produced by primer combination E-AGG/M-CTT in O. niloticus and E-ACA/M-CAC in the Ni-ao, which would work as molecular markers to identify O. niloticus, O. aureus and their hybrids F1.
|
|
Received: 08 July 2006
|
|
|
|
|
|
