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Abstract The transformation system by electroporation of the biocontrol bacteria ANTI-8098A (Bacillus cereus) was optimized in this paper. The results showed that the system was best for the electroporation when the concentration of compentent cell was 1.14×108 CFU/mL and the content of plasmid was 118 ng. The proportions of the green cells decreased when the strains of ANT8098A:pCM20 was cultured in the non-selective medium. And the growth speed of gfp-tagged strains was almost the same to the wild type strain ANTI-8098A. The best culture conditions of wild type strain and gfp-tagged one including the culture temperature of 35 ℃ and rotation speed of 250 r/min were almost the same. Both strains ANTI8098A and ANTI8098A: pCM20 showed the same in vitro inhibition of Ralstonia solanacearum from different crops.
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Received: 17 July 2009
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