Abstract In the present study, the effect of different concentrations and different adding proposal of rhodiola polysaccharide on freezing boar spermatozoa was tested. Results: 1. rhodiola polysaccharide could provide better protective action for boar spermatozoa during cryopreservation, and especially anti-oxidation within the cooling period; 2. the effect of rhodiola polysaccharide added inⅠextender was better than that of added in Ⅱextender, and the effective concentration of rhodiola polysaccharide was 6 mg/L.
