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Abstract In our previous study, we had successfully obtained normal mice derived from oocytes following intracytoplasmic fresh sperm injection (ICSI). In the current study, we tested the developmental potency of ICSI embryos produced using freeze-dried sperm. The sperm of B6D2F1 mice were firstly freeze-dried in Tris-HCl-EDTA（pH8.2）solution for 4 h, then were individually injected into mature KM oocytes. Six hours later, 83.0% of fertilized eggs shown two pronuclei and extruded the 2nd polar body. The development rates of these fertilized embryos at the 2-cell (92.0% vs 99.5%), 4-cell (52.7% vs 97.2%), morulae (36.6% vs 86.3%) and blastocyst stages (21.4% vs 68.7%), were significantly (p<0.01) lower than that of the embryos fertilized by fresh sperm injection. After transferred to pseudo-pregnant KM female mice, 63.0% (17/27) of the 2-cell embryos were implanted on D10 and 21.7% (13/60) of them developed to term. Eleven pups developed into normal and fertile adult. Here we reported that the first birth of mouse offspring following ICSI using freeze-dried sperm in China. It was valid to store mammalian sperm using freeze-dried procedure.
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Received: 19 July 2006
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