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| The expression of S1 gene of IBV in E.coli and the original identification of it’s biological activity |
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Abstract Abstract: The S1 gene of IBV was cloned into expression vector pGEX-6p-1 by gene recombination technology. E. coli transformed by the recombinant plasmid containing IBV S1gene was induced by IPTG. after the induced A E. coli were lysed, fusion proteins in pGEX-IBV-S1A and pGEX-IBV-S1B were revealed by SDS-PAGE. And, obvious bands in pGEX-IBV-S1A and pGEX-IBV-S1B were showed by Western-blot. The result demonstrated partial antigenicity of the expression products. GST fusion proteins of A and B were purified using Glutathione Sepharose 4B. Rabbits were vaccinated with the purified GST fusion proteins. The antibody and virus were incubated in 37℃ incubator for 30 minutes, and then TOC experiment was made. The result showed that the antibody induced by segment A could inhibit the partial activity of IBV with SN endpoint of 1:18 while the antibody induced by segment B could completely inhibit the activity of virus with SN endpoint of 1:53. It is proved that the immunogenicity of segment B was better than that of segments A.
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Received: 20 March 2006
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