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| Isolation and Identification of Calicivirus from Mink |
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Abstract In June 2007, an infectious disease outbreak was occured at a mink（Mustela vison）farm in Dalian, China. The sick minks developed clinical symptoms of ocular discharges , nasal-dry and others, and some minks died from the diseases. A virus was isolated from collected samples of nasal swabs, ocular swabs and other organs. The virus isolate grew well in Vero cell and other cell cultures. Identification of the virus isolate was conducted by a series of methods and procedures, such as morphological observation, physical and chemical analysis, identification of the type of nucleic acid and nucleic acid sequence analysis. Physicochemical identification results show that the virus has resistance to chloroform, ethyl ether and sodium deoxycholate, can tolerate the acidic environment till pH3.0, reserves infection capacity at 50℃ for 1 hour, and does not sensitive to trypsin. Virus morphology assay results show that the diameter of the virus is 30 ~ 35nm, no capsule. Nucleic acid identified it as RNA-type virus. Nucleic acid sequence analysis with the GenBank recorded on the United States in 1980 isolated from the mink calicivirus（MCV） AF338405, AF338406 and AF338407 showed that the nucleotide homology is 99.3% ~ 99.5%, and amino acid homology is 95.4% ~ 100%. The results suggested that the virus isolate from the died mink is a Mink calicivirus. It is the first time to report the isolation and identification of the calicivirus from mink in China.
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Received: 04 February 2009
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