|
|
|
| Cloning of Carnation GA20-oxidase Gene and Construction of Plant RNAi Vector |
|
|
|
|
Abstract A pair of degenerate primers were designed based on the conserved domain of the GA 20-oxidase reported in other plants. A full-length cDNA (1 179 bp) of carnation (Dianthus caryophyllus L. cv. Marster) GA20-oxidase named Dc20ox was cloned by RT-PCR and RACE methods. The deduced amino acids of this sequence shared a high homology. Blast showed that the amino acids sequence presented a high homology (66%~75%) with the GA20-oxidase in other plants. A RNAi vector (pART400) was constructed by a high homology 400 bp fragment.
|
|
Received: 02 April 2008
|
|
|
|
|
|
|
