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Spatio-temporal Expressed Analysis of Two Kinds of Chemical Communication
Related Proteins in Working Bee of Apis cerana cerana |
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Abstract The spatio-temporal expressed profiles of two kinds of chemical communication related to proteins genes of Apis cerana cerana: odorant-binding protein of Ac-ASP2 and chemosensory protein of Ac-ASP3, were identified by Real-time PCR. The results with the method of 2-ΔΔCt showed that Ac-ASP2 was a gene coding antenna specific protein, which did not express in larva and pupa, but had such discontinuous high abundance periods: 1, 9, 15, 27and 30 d, the expressing abundance at such periods was tenfold higher than that at other periods. From the distribution of Ac-ASP3 mRNA in different issues, the transcript amounts seemed to be higher in wings, abdomen and thorax (about 106 order of magnitude), and lower in legs, antennae and head (about 105 order of magnitude), the special relation of the original copy number was: wings >abdomen >thorax >legs >antennae >head. The results above showed that Ac-ASP3 had intimate relation with chemosensory behavior of wings and abdomen in Apis cerana cerana.
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Received: 22 April 2008
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