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Abstract In this study，ISSR conditions on pine caterpillars were optimizatied.The factors which affected the ISSR results of pine caterpillars were studied.The results showed that 2.5mmol.L-1 Mg2+，0.8μmolL-1 primer, 0.20mmol. L-1dNTP，1.25ＵTag polymerase，15～30 ng template DNA and the optimal annealing temperature of primers of 45.9～52.4℃ in 25μL ISSR reaction system were the best. Though above PCR system，ISSR fragments of 14 populations from different district of 4 species were obtained and detected.Polymorphism between different populations was abundantly detected by 1.5％ arognose which showed this system was suitable and stable.
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Received: 25 December 2006
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