Abstract In order to cloning the tandem inhibin gene, the two different primers were desigened according to the Genbank sequence. p1INH and p2INH were Tandem through XbaⅠ. Doubled gene encoding porcine inhibinα(1～32) was cloned and recombined with pcDNA3.1 expression vector to construct inhibin gene vaccine pcINH. The recombinant plasmid pcINH was expressed in E. coli. DH5α.The expression protein had inhibin immunization activity and has no homology with other protein in the body.
