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Abstract A gene encoding β-endoglucanase (EG) from Vibrio fischeri EM17 was amplified by PCR and inserted into E.coli expression plasmid pGEX-6p-1.The recombinant plasmid was transformed into E.coli BL21 and induced to express. Sequecing analysis showed that the sequence of β-endoglucanase gene fragment exhibited 97.1％ homology with Vibrio fischeri ES114’s ,while it was very low compared with other organisms’ .The optimal enzyme reaction temperature is 60℃, and the optimal pH is 9.0. The result of the effect of ion on enzyme activity showed that the enzyme activity relied on metal ion, Mg2+and Ca2+ showed a stimulating effect while Mn2+ and Pb2+ showed an strong inhibitory effect.
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Received: 06 April 2007
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